upa selective inhibitor uk122  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Modeling the role of urokinase plasminogen activator, uPA, and circulating Cancer-Associated Fibroblasts (cCAFS) in breast cancer cell extravasation

doi: 10.1101/2025.06.11.659108

Figure Lengend Snippet: A. Western blot showing that CAF23 conditioned media increases uPA protein in MDA-MB231 cells. B. qPCR of PLAU in MDA231 or DT28 cells treated for 3 days with non-targeting siRNAs (siNT) or PLAU siRNAs (siPLAU). PLAU was normalized to the 18S ribosomal RNA. Inset: Western blot of siNT or siPLAU MDA-MB231 cells. C. Endothelial binding assay of MDA-MB231, DT28 and SUM159 cells treated for 3 days with siNT or siPLAU. D. Time course of endothelial binding assay using MDA-MB231 cells treated with siNT or siPLAU showing % of cells bound for the indicated periods. E. Endothelial binding assay using MDA-MB231 cells pre-treated with vehicle or uPA inhibitor, UK122, for 24 hrs at increasing concentrations. * p ≥ 0.05, ** p ≥ 0.005, *** p ≥ 0.0005

Article Snippet: The following chemicals were used in our studies: Bovine Hyaluronidase (StemCell Technologies), 4MU (Sigma Aldrich), UK122 (MedChemExpress), TGFβ1 (R and D Systems), Cell Tracker Deep Red (Invitrogen, C34565) or Cell Tracker Red (Invitrogen, C34552), FITC-lectin (Vector Laboratories).

Techniques: Western Blot, Binding Assay

Journal: Scientific reports

Article Title: Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice.

doi: 10.1038/s41598-023-29824-1

Figure Lengend Snippet: Figure 5. Effect of uPA inhibitor on DSS-induced colitis in C57BL/6 J mice. (A) Male mice were administered 2% DSS in their drinking water for 1 week, and intraperitoneally injected with 2 mg/kg or 4 mg/kg UK122 or vehicle from day 1 to day 7 (n = 18 per group). They were sacrificed at day 8. (B) Representative macroscopic images of the colon in each group. The lower right panel represents a colorectum of mice that did not receive DSS. (C) DAI was evaluated at day 8 by the scoring method of Cooper et al.10 (D) Representative H&E staining images of colon sections in each group. The lower right panel represents a colorectum of mice that did not receive DSS. Scale bar, 100 μm. (E) Histologic score of H&E staining image was evaluated by the scoring method of Williams et al.11 Data represent mean ± SEM. *p < 0.05, **p < 0.01 by Dunnett’s multiple comparison test.

Article Snippet: The uPA-selective inhibitor UK122 (Santa Cruz Biotechnology, Dallas, TX) was dissolved in dimethyl sulfoxide (DMSO) and saline.

Techniques: Injection, Staining, Comparison

Journal: Scientific reports

Article Title: Urokinase-type plasminogen activator blockade ameliorates experimental colitis in mice.

doi: 10.1038/s41598-023-29824-1

Figure Lengend Snippet: Figure 6. Cytokine expression in colorectal tissue from DSS-treated uPA−/− mice, and C57BL/6 J mice intraperitoneally injected with UK122. (A) Cytokine concentration in the colorectal extracts of WT (n = 8) and uPA−/− (n = 10) mice measured using Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit in triplicate. (B) Cytokine concentration in the colorectal extracts of 8 mice treated with 2 mg/kg or 4 mg/kg UK122 or vehicle measured using Bio-Plex Pro Mouse Cytokine 23-Plex Assay kit in duplicate. Data represent mean ± SEM. *p < 0.05, by Student’s t test (A) and Dunnett’s multiple comparison test (B).

Article Snippet: The uPA-selective inhibitor UK122 (Santa Cruz Biotechnology, Dallas, TX) was dissolved in dimethyl sulfoxide (DMSO) and saline.

Techniques: Expressing, Injection, Concentration Assay, Plex Assay, Comparison

Journal: BMC Cancer

Article Title: Identification of genes regulating migration and invasion using a new model of metastatic prostate cancer

doi: 10.1186/1471-2407-14-387

Figure Lengend Snippet: The role of EpCAM, integrin β4 and uPA in migration and invasion of DU145-LN4 cells. Cell migration of DU145-LN4 cells in a transwell migration assay (A-C) and a Matrigel invasion assay (D-F) . siRNA knockdown of (A) EpCAM, (B) integrin β4, or (C) uPA significantly inhibited cell migration relative to control siRNA and untreated cells. siRNA knockdown of (D) EpCAM or (E) ITGB4 did not significantly affect cell invasion in a Matrigel invasion assay. (F) uPA siRNA significantly inhibited cell invasion relative to control siRNA and untreated cells. Western blot analysis of whole cell lysates from cells treated with siRNA in parallel with migration/invasion assays: (G) EpCAM, (H) integrin β4, and uPA western blots. Blots were probed with GAPDH as loading controls. (I) Western blot analysis of whole cell lysates from cells treated with control siRNA or si-uPA and serum starved (−) or serum stimulated (+). p-AKT and p-S6K were induced after serum addition in control cells but not in cells lacking uPA. Total AKT, S6K, and GAPDH served as controls. DU145-LN4 cell migration (J) , and cell invasion (K) were significantly inhibited by treatment with 10 μM amiloride or 10 μM UK122. Students t-test, *p ≤ 0.05, **p ≤ 0.01, n.s.-not significant. Top asterisked p value represents analysis between untreated cells and specific siRNA, lower value indicates analysis between control siRNA treated cells and specific siRNA.

Article Snippet: For uPA inhibitor experiments, cells were treated with 0.1% DMSO vehicle, 10 μM amiloride or UK122 (EMD Millipore, Billerica, MA).

Techniques: Migration, Transwell Migration Assay, Invasion Assay, Western Blot